Fuse-It-mRNA

Optimized product to transfer mRNA into the cytoplasm of living cells

 

Technical FeaturesFuse-It-N_mRNA

  • Fusogenic liposomes are used as carriers for the transfer of mRNA into the cytoplasm of a wide range of mammalian cells
  • Based on very short incubation times, the transfer of mRNA occurs in a very mild fashion
  • Highly efficient, also in non-proliferating cells (e.g. neurons or cardiomyocytes)
  • Fusion processes are completed within 5 to 20 minutes with superior biocompatibility
  • mRNA translation starts directly after fusion: No endocytosis or lysosomal degradation; no gene transfer into the nucleus needed
  • Protein synthesis is detectable after 2 hours

Applications

  • mRNA translation and degradation studies
  • Protein biochemistry studies: synthesis, folding, processing, stability, localization, degradation
  • Beneficial alternative to transient plasmid transfections -> no biosafety lever required and no risk of genomic intergration

Two hours after fusion (efficiency of 80-100%, independent of cell type used) first protein signal is already detectable and can be analyzed.

Fuse-It-N-mRNA-sample-CHO

 Fusion efficiency after the transfer of eGFP-mRNA in CHO cells using Fuse-It-mRNA:

In just 2 hours after starting the experiment, the fusion efficiency is 80-100%, and GFP-mRNA is already detectable in the cells. The eGFP amount increases and remains stable for more than 3 days

Fuse-It-mRNA vesicles were filled with eGFP-mRNA and fused for 10 minutes with CHO-K1 cells. (red = visualized fusion, green = green fluorescent protein)

Ordering information

Cat. No. Description Pcs./Box
60500 Fuse-It-mRNA, infrared fluorescent: 2 x 150 µl solution 1
60501 Fuse-It-mRNA, infrared fluorescent: 2 x 300 µl solution 1

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