NEW PRODUCT Fuse-It-siRNA

Optimized fusion product to transfer siRNA into living cells for rapid and efficient gene silencing

 

  • Exceptionally efficient siRNA transfection, especially in primary and non-dividing cells, such as keratinocytes
  • Extremely low cytotoxicity
  • Fastest siRNA delivery—no endocytosis or lysosomal degradation

 

Technical Features

  • Lipofection-independent transfer of siRNA into living cells
  • Fast siRNA transfection into the cytoplasm: no endocytosis, no lysosomal degradation, and no gene transfer to the nucleus
  • siRNA transfer completed within 5-20 minutes
  • Small amount of siRNA is sufficient for effective knockdown
  • Excellent biocompatibility because of low cytotoxicity
  • siRNA transfer into cells without generating genetically modified organisms (GMO)

 

Applications

  • Gene silencing / Protein knockdown

 

Fuse-It-siRNA Results in Extremely Fast siRNA Transfer and Highly Efficient Knockdown of GFP

 

Transiently GFP-expressing CHO-K1 cells were fused with Fuse-It-siRNA vesicles containing Silencer® GFP (eGFP) siRNA (Thermo Fisher Scientific) for 8 minutes. In contrast to the control cells, GFP was almost completely diminished in Fuse-It-siRNA-treated cells 18 hours after the transfer.

 

Membrane Fusion – The Direct Path to Gene Silencing

 

ORDER NOW

INSTRUCTIONS

PRODUCT FLYER

 

 

 

Kommentare sind geschlossen