Fuse-It-L

Optimized product to incorporate lipids or amphipathic molecules into the plasma membrane of living cells by fusion

R_6021X_FuseIt_L_RGB

Technical Features

  • Transfer of lipids and amphipathic molecules into the cell plasma membrane by the use of fusogenic liposomes
  • Superb fusion efficiency of 80 to 100% independent of the cell type used
  • Fusion process completed within 1 to 10 minutes with superior biocompatibility

Applications

  • Lipid imaging for turnover, traffic, and functional analyses in living cells
  • Lipid raft, lipid microdomain, and lipid diffusivity measurements in living cells
  • Blockade, induction, or replacement of lipid induced/regulated signal cascades
  • Re-incorporation of lipids into mutant cells whose lipid biosynthesis is altered

The cells can be analyzed immediately after fusion has taken place. If necessary more than just one lipid of interest can be incorporated at once. Depending on experimental needs, fusion can be detected by fluorescence.

Fuse-It-LBodipy FL C12-galactocerebroside and TopFluor cholesterol were incorporated into the plasma membrane of CHO cells using Fuse-It-L after 5 minutes of incubation.

Ordering information

Cat. No. Description                                                                         Net price excl. value added tax
60210 Fuse-It-L, lyophilized, for 100 μl solution, IR fluorescent 160 €
60211 Fuse-It-L, lyophilized, for 4 x 25 μl solution, IR fluorescent 180 €

Specifications/Content

Concentration             3 mM

Storage                       -20°C

Shelf life                     6 months as solution

ExMax/EmMax             750/780 nm

Instructions

MSDS Fuse-It-L

ORDER NOW

Comments are closed.