Fuse-It-mRNA for mRNA Transfection

A fusion reagent for rapid mRNA transfection into the cytoplasm of living cells

Technical Features

  • Immediate mRNA translation leads to protein synthesis detectable after only 15-30 minutes
  • Beniag’s fusion mechanism works perfectly in proliferating cells as well as in non-proliferating cells (e.g. cardiomyocytes)
  • Highly efficient and biocompatible, especially in primary cells (e.g., neurons or HUVECs) and stem cells

Applications

  • mRNA translation and degradation studies
  • Protein biochemistry studies: synthesis, folding, processing, stability, localization, degradation
  • mRNA transfer into primary cells without generating genetically modified organisms (GMO)
  • Genome engineering by RNA-only CRISPR/Cas technology

Technical Features

  • Lipofection-independent transfer of mRNA into living cells
  • the transfer of mRNA is completed within 5–20 minutes
  • No endocytosis involved
  • No lysosomal degradation needed
  • without transfer to the nucleus
  • Protocol optimized for the transfer of functionally capped and polyadenylated mRNA
  • No Biosafety Laboratory Level 1 or 2 necessary
  • Excellent biocompatibility with low cytotoxicity

Membrane Fusion – The Direct Way to Protein Expression

The novel mRNA transfection reagent Fuse-It-mRNA uses fusogenic liposomes for mRNA transfer without endosomal release

Efficiency of mRNA Expression in Various Cell Types After Treatment with Fuse-It-mRN

Celltype overview

Cell Type Organism Incubation Time Efficiency of mRNA Expression*
Primary Cells
nHEK, foreskin human 8 min 70–90%
Cortical neurons, embryonic (adherent) rat 10 min 40–60%
HFF human 10 min 50–70%
Cell Lines
A549 human 10 min 80–100%
CHO-K1 hamster 10 min 80–100%
Beas-2B human 8 min 80–100%
3T3 murine 8 min 80–100%
MEF wildtype murine 10 min 70–90%
HT-1080 human 10 min 80–100%
HUH-7 human 8 min 70–90%
MCF-10A human 10 min 70–90%
Rat1 rat 10 min 80–100%
THP-1 (suspension) human 3 min 70–90%

* The mRNA expression efficiency may vary, depending on further experimental factors (e.g., culture medium, cell fitness, passage number, and protocol optimization).

Ordering information

Cat. No. Description

Net price excl.

value added tax

 
60505 Fuse-It-mRNA, infrared fluorescent: 2 x 150 µl solution 230 €  
60506 Fuse-It-mRNA, infrared fluorescent: 2 x 300 µl solution 400 €  

Instructions

Short Protocol

MSDS Fuse-It-mRNA FS

MSDS Fuse-It-mRNA NB

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